Development of an accessible and low-cost micro-fluidic system (lab-on-a-chip) for detecting circulating breast cancer tumor cells

Authors

  • Julio Valdivia-Silva Departamento de Bioingeniería e Ingeniería Química, Universidad de Ingeniería y Tecnología – UTEC. Lima, Perú. http://orcid.org/0000-0002-7061-3756
  • Luz Pérez-Tulich Departamento de Bioingeniería e Ingeniería Química, Universidad de Ingeniería y Tecnología – UTEC. Lima, Perú. https://orcid.org/0000-0003-4652-067X
  • Luis Flores-Olazo Departamento de Bioingeniería e Ingeniería Química, Universidad de Ingeniería y Tecnología – UTEC. Lima, Perú. https://orcid.org/0000-0002-0754-0849
  • Marco Málaga-Julca Departamento de Bioingeniería e Ingeniería Química, Universidad de Ingeniería y Tecnología – UTEC. Lima, Perú. https://orcid.org/0000-0002-6477-8423
  • Adolfo Ubidia Departamento de Bioingeniería e Ingeniería Química, Universidad de Ingeniería y Tecnología – UTEC. Lima, Perú. https://orcid.org/0000-0001-6980-526X
  • Aaron Fleschman Lerner Research Institute, Biomedical Engineering Department, Cleveland Clinic. Cleveland, Ohio, Estados Unidos. https://orcid.org/0000-0002-6128-3425
  • Heinner Guio Departamento de Bioingeniería e Ingeniería Química, Universidad de Ingeniería y Tecnología – UTEC. Lima, Perú; INBIOMEDIC Research and Technological Center. Lima, Perú. https://orcid.org/0000-0003-0078-1188

DOI:

https://doi.org/10.35663/amp.2020.371.967

Keywords:

Diagnostic equipment, Circulating tumor cells, Magnetics, Breast cancer

Abstract

Objective: to develop a microfluidic system (lab-on-a-chip) for detecting circulating breast cancer tumor cells. Materials and methods: the device was designed using 3D technology, and it was manufactures using soft photolithography and a laser cutting machine. The system performance and its magnetic settings were assessed using Jurkat cells and breast cancer cells that show different expression of CD45 and EpCAM surface markers. Antibodies against these markers were bound to magnetic pellets. Additionally, iron nanoparticles were used for assessing their entrapment. Results: nanoparticles were significantly trapped in the area set by magnetic field modeling. Tumor cells labeled with magnetic antibodies became trapped. Conclusions: we were able to manufacture a lab-on-a-chip system that is capable to trap circulating breast cancer tumor cells, which may become an excellent tool for diagnosis and follow-up for this condition.

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Published

2020-03-31

Issue

Section

ORIGINAL ARTICLES

How to Cite

1.
Development of an accessible and low-cost micro-fluidic system (lab-on-a-chip) for detecting circulating breast cancer tumor cells. Acta Med Peru [Internet]. 2020 Mar. 31 [cited 2024 Nov. 4];37(1). Available from: https://amp.cmp.org.pe/index.php/AMP/article/view/967